Please confirm that an ethics committee approval has been applied for or granted: Not relevant

Background and Aims Antimicrobial resistance(AMR) has become a global issue. Not only decreasing the treatment options but a serious threat to low-income countries associated with both misuse of antibiotics. Antibiogram is essential for a hospital to track changes in AMR and to guide empirical antimicrobial therapy. The goals of the current study is to ascertain burden of nosocomial infections in ICU patients, pinpoint involved pathogens, and sensitivity to antimicrobial drugs.

Methods This study was retrospective cross-sectional in nature. Study was conducted at SICU of Doctors Hospital and Medical Center, Lahore over a period of one year from January 2021 to December 2021. Patients of both genders with age above 18years were included in this study. 364 patients were recorded for the purpose of study and analyzed. All cultures were processed in accordance with standard microbiological protocols defined by CLSI.The antibiogram was constructed according to CLSI and Stanford University’s web-based antibiogram.

Results Among 364 patients analyzed in the study, the cultures obtained from different sites were Blood (54%), Urine (33%) and tracheal (13%). Among blood cultures, no organism was isolated. E coli was most common organism among urine and Klebsiella Pneumonia was most frequently encountered in tracheal cultures. Vancomycin and Linezolid showed zero percent resistance to Staphylococcus. Collistin showed zero percent resistance for Acinetobacter and Klebsiella.

Conclusions The high frequency of multidrug resistance bacteria in ICU suggests that we need to prescribe broad-spectrum antibiotics more wisely to reduce pressure on sensitive strains. Vancomycin and Linezolid for Gram positive organisms and Collistin for Gram negative organisms.