Article Text

Download PDFPDF

B173 Local anesthetics affect tumor biology in an ex vivo tissue model of non-small cell lung cancer
  1. J Krömer1,2,
  2. NA Hoang2,
  3. D Sittig2,
  4. C Welling2,
  5. D Junk2,
  6. J Mölleken2,
  7. J Zönnchen2,
  8. A Monecke3,
  9. S Krämer4,
  10. S Kallendrusch2 and
  11. T Piegeler1
  1. 1University Hospital Leipzig, Department of Anesthesiology and Intensive Care, Leipzig, Germany
  2. 2University of Leipzig, Institute of Anatomy, Leipzig, Germany
  3. 3University of Leipzig, Institute of Pathology, Leipzig, Germany
  4. 4University Hospital Leipzig, Department of Visceral, Transplant, Thoracic and Vascular Surgery, Leipzig, Germany


Background and Aims Rates of tumor-related death in lung cancer are still high, despite improved treatment options and a deeper understanding of tumor biology and immune responses. Perioperative use of local anesthetics might influence outcome after tumor surgery(1), an effect possibly based on anti-inflammatory properties, leading to an inhibition of signaling processes crucial for metastasis(2–4). However, effects of local anesthetics on tumor biology in “real-life” tumors has not been investigated yet.

Methods After surgical removal, tumor slices from patients with NSCLC were cultured ex vivoand treated with different concentrations (1µM/10µM) of ropivacaine or lidocaine in absence or presence of cisplatin (3µM, n=9). After 72 h tissues were analyzed using immune-histochemistry/-fluorescence and Western blot. Levels of Ki67 (proliferation), cPARP (apoptosis), intercellular-adhesion-molecule-1 (ICAM-1), and CD163 expression (in tumor-associated macrophages) were assessed. The study was approved by the ethics committee at the University of Leipzig (protocol number 370/13-ek) and funded by the ESRA Research Grant.

Results Ropivacaine reduced the proliferating tumor cell fraction by 20% (1µM) and 27% (10µM), respectively. Both ropivacaine and lidocaine increased the share of apoptotic tumor cells in a dose-dependent manner. Expression patterns of ICAM-1 as well as of tumor associated macrophages (CD163) were altered by lidocaine and ropivacaine in immunofluorescence staining.

Conclusions Local anesthetics might alter tumor-cell biology and the tumor-microenvironmentex vivo. Proliferation and ICAM-1 expression of tumor cells, along with CD163 expression of macrophages were affectedby local anesthetics, thus supporting aless invasive and less malignant tumor phenotype. This once more underlines the possible impact of these drugs on patient outcome after tumor surgery.

Statistics from

Request Permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.