Article Text

Download PDFPDF
ESRA19-0131 Colonization of epidural catheters seen through the electron microscope
  1. G Van Samkar1,
  2. P Balraadjsing2,
  3. H Hermanns1,
  4. I Hoogendijk2,
  5. M Hollmann1,
  6. B Zaat2 and
  7. M Stevens1
  1. 1Amsterdam UMC University of Amsterdam, Department of Anesthesiology, Amsterdam, the Netherlands
  2. 2Amsterdam UMC University of Amsterdam, Department of Medical Microbiology, Amsterdam Infection and Immunity Institute, Amsterdam, the Netherlands


Background and aims Epidural catheters are frequently colonized by gram positive bacteria. the incidence of epidural infections is low, but their consequences can be devastating. We investigated the pattern of bacterial colonization of epidural catheters. Epidural catheters of patients showing signs of local infection were investigated by scanning electron microscopy (SEM) in order to delineate mechanism of catheter infection.

Methods 28 patients receiving thoracic epidural analgesia for more than 72 hours were studied. All received antibiotic prophylaxis after catheter placement (cefazolin, metronidazole). Skin swabs and catheter segments were cultured after catheter removal. Bacteria were identified by matrix-assisted laser desorption/ionization time of flight mass spectrometry. SEM of culture positive catheters was performed.

Results 27 of 28 catheters could be investigated. Colonization percentages were: skin swab in 22%, extracorporeal segments in 11% and subcutaneous segments in 3.7%. Cultures revealed Staphylococcus epidermidis, Staphylococcus simulans, Propionibacterium acnes, and Micrococcus luteus. In one patient, a clinically relevant infection was recorded, caused by S. epidermidis. Bacteria were cultured from extracorporeal and subcutaneous segments and the tip of this catheter. SEM displayed host cell-like structures on inner catheter surface and an intraluminal fibrin network which formatted around a catheter hole.

Conclusions Epidural catheter colonization seems to spread from the skin segments to the deeper parts of the catheter, resembling the pattern of colonization of central venous catheters. the inner surface of the catheter tip is invaded by probably immune cells and fibrin fibers forming a microfilm-like structure.

Statistics from

Request Permissions

If you wish to reuse any or all of this article please use the link below which will take you to the Copyright Clearance Center’s RightsLink service. You will be able to get a quick price and instant permission to reuse the content in many different ways.